ecori restriction enzymes (New England Biolabs)
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ecori restriction enzymes
Ecori Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1617 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nhei+hf/NheI-HF/pm42036043-165-42-48
Average 96 stars, based on 1617 article reviews
Ecori Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1617 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nhei+hf/NheI-HF/pm42036043-165-42-48
Average 96 stars, based on 1617 article reviews
ecori restriction enzymes - by Bioz Stars,
2026-09
96/100 stars
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other:Article Title: Nonsense-mediated mRNA decay orchestrates neuronal migration and cortical lamination while modulating Reelin and ciliary gene regulatory networks Article Snippet: Western Blot:Article Title: Screening assay to monitor mono-ADP-ribosylhydrolase activity of viral macrodomains in cells Article Snippet: The following reagents were used in this study: ß-NAD + (Sigma-Aldrich, N6522), 32 P-NAD + (Hartmann, FP-321), Protease inhibitor cocktail (Sigma-Aldrich, P8340; MedChem Express, HY-K0011), polybrene (Hexadimethrinbromid, Sigma Aldrich, 107689), doxycycline (Sigma-Aldrich, D9891), Z8539_0023 (Enamine-ID: Z5010894404; 4-hydroxy-1-{4-[(phenylcarbamoyl)amino]benzamido}-2.3-dihydro-1H-indene-2-carboxylic acid), Z8539_0029 (Enamine-ID: Z5010894407; 1-{4-[(cyclopropylcarbamoyl)amino]benzamido}-2,3-dihydro-1H-indene-2-carboxylic acid), Z3122 (Enamine-ID: Z5389393122; 3-(4-bromophenyl)-3-({7H-pyrrolo[2,3-d]pyrimidin-4-yl}formamido)propanoic acid), LL1_0023 (Enamine-ID: Z5030903496, 8-fluoro-6-{7-fluoro-9H-pyrimido[4,5-b]indol-4-yl}-6-azaspirol[3.4]octane-8-carboxylic acid), LRH-0003 (Enamine-ID: Z5265428218, 1-({7-fluoro-9H-pyrimido[4,5-b]indol-4-yl}amino)pyrrolodin-2-one), Z8539 (Enamine-ID: Z4718398539, 1-{4-[(cyclopropylcarbamyol)amino]benzamido}-4-hydroxy-2,3-dihydro-1H-indene-2-carboxylic acid) ; OUL232 (MedChem express HY-148566), OUL 243 ; GS441524 (Tocris, #7227, (2 R ,3 R ,4 S ,5 R )-2-(4-Aminopyrrolo[2,1- f ][1,2,4]triazin-7-yl)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-carbonitrile); MDOLL-0229 ; the pyrollo-pyrimidine-based compounds (7 H -pyrrolo[2,3- d ]pyrimidin-4-yl)- L -tryptophanate ( 5a ), (7 H -pyrrolo[2,3- d ]pyrimidin-4-yl)- L -tryptophanate ( 5c ), ( S )-2-((7 H -pyrrolo[2,3- d ]pyrimidin-4-yl)amino)- N -((2-chloropyridin-4-yl)methyl)-3-(1 H -indol-3-yl)propenamide ( 6b ), ( S )-2-((7 H -pyrrolo[2,3- d ]pyrimidin-4-yl)amino)-3-(1 H -indol-3-yl)- N -(pyridin-2-ylmethyl)propenamide ( 6d ), ( R )-2-((7 H -pyrrolo[2,3- d ]pyrimidin-4-yl)amino)-3-(1 H -indol-3-yl)- N -(pyridin-2-ylmethyl)propenamide ( 6e ) . .. TALON metal affinity resin (Takara, 635501), anti-GFP (Rockland, 600-101-2015, WB: 1:2000), anti-gamma-Tubulin (Sigma-Aldrich, GTU88, WB: 1:2000), anti-GAPDH (Santa Cruz, sc-32233, WB 1:2000), anti-PARP15 (Proteintech, 18126-1-AP, WB: 1:2000), anti-FLAG (Sigma Aldrich, F3165, clone M2, WB: 1:5000, IF: 1:500), goat-anti-mouse-HRP (Jackson Immunoresearch, 115-036-068, WB: 1:10000), rabbit-anti-goat-HRP (Santa Cruz, sc-2768, WB: 1:5000), goat-anti-rabbit IgG (Cell Sinalling, 7074P2, WB: 1:2000), goat-anti-mouse-Alexa-Flour-633 (Invitrogen, A21052, IF: 1:500), Spy650-DNA (tebu-bio, sc501, 1:1000), Immu-Mount (Epredia, 9990402), Hoechst33258 (Sigma-Aldrich, 94403), Lipofectamine 3000 (Thermo Scientific, L3000001), NEBuilder HiFi°DNA°Assembly°Master Mix (NEB, E2621L), CloneAamp TM HiFi PCR Premix (Takara Bio, 639298), GatewayTM BP ClonaseTM II Enzym-Mix (Thermo Scientific, 11789020), GatewayTM LR ClonaseTM II Enzym-Mix (Thermo Scientific, 11791020), AflII (New England Biolabs, R0520S), Article Title: Screening assay to monitor mono-ADP-ribosylhydrolase activity of viral macrodomains in cells. Article Snippet: The following reagents were used in this study: ß-NAD+ (Sigma-Aldrich, N6522), 32P-NAD+ (Hartmann, FP-321), Protease inhibitor cocktail (Sigma-Aldrich, P8340; MedChem Express, HYK0011), Polybrene (Hexadimethrinbromid, Sigma Aldrich, 107689), Doxycycline (Sigma-Aldrich, D9891), Z8539_0023 (Enamine-ID: Z5010894404; 4-hydroxy-1-{4[(phenylcarbamoyl)amino]benzamido}-2.3-dihydro-1H-indene-2-carboxylic acid), Z8539_0029 (Enamine-ID: Z5010894407; 1-{4-[(cyclopropylcarbamoyl)amino]benzamido}-2,3-dihydro-1Hindene-2-carboxylic acid), Z3122 (Enamine-ID: Z5389393122; 3-(4-bromophenyl)-3-({7H- AR TI CL E IN P RE SS pyrrolo[2,3-d]pyrimidin-4-yl}formamido)propanoic acid), LL1_0023 (Enamine-ID: Z5030903496, 8-fluoro-6-{7-fluoro-9H-pyrimido[4,5-b]indol-4-yl}-6-azaspirol[3.4]octane-8-carboxylic acid), LRH-0003 (Enamine-ID: Z5265428218, 1-({7-fluoro-9H-pyrimido[4,5-b]indol-4yl}amino)pyrrolodin-2-one), Z8539 (Enamine-ID: Z4718398539, 1-{4- [(cyclopropylcarbamyol)amino]benzamido}-4-hydroxy-2,3-dihydro-1H-indene-2-carboxylic acid)25; OUL232 (MedChem Express HY-148566), OUL 243 40; GS441524 (Tocris, #7227, (2R,3R,4S,5R)-2-(4-Aminopyrrolo[2,1-f][1,2,4]triazin-7-yl)-3,4-dihydroxy-5(hydroxymethyl)tetrahydrofuran-2-carbonitrile); MDOLL-0229 47; the pyrollo-pyrimidine-based compounds (7H-pyrrolo[2,3-d]pyrimidin-4-yl)-L-tryptophanate (5a), (7H-pyrrolo[2,3-d]pyrimidin4-yl)-L-tryptophanate (5c), (S)-2-((7H-pyrrolo[2,3-d]pyrimidin-4-yl)amino)-N-((2-chloropyridin-4yl)methyl)-3-(1H-indol-3-yl)propenamide (6b), (S)-2-((7H-pyrrolo[2,3-d]pyrimidin-4-yl)amino)-3(1H-indol-3-yl)-N-(pyridin-2-ylmethyl)propenamide (6d), (R)-2-((7H-pyrrolo[2,3-d]pyrimidin-4yl)amino)-3-(1H-indol-3-yl)-N-(pyridin-2-ylmethyl)propenamide (6e) 30. .. TALON metal affinity resin (Takara, 635501), anti-GFP (Rockland, 600-101-2015, WB: 1:2000), anti-gamma-Tubulin (SigmaAldrich, GTU88, WB: 1:2000), anti-GAPDH (Santa Cruz, sc-32233, WB 1:2000), anti-PARP15 (Proteintech, 18126-1-AP, WB: 1:2000), anti-FLAG (Sigma Aldrich, F3165, clone M2, WB: 1:5000, IF: 1:500), goat-anti-mouse-HRP (Jackson Immunoresearch, 115-036-068, WB: 1:10000), rabbitanti-goat-HRP (Santa Cruz, sc-2768, WB: 1:5000), goat-anti-rabbit IgG (Cell Sinalling, 7074P2, WB: 1:2000), goat-anti-mouse-Alexa-Flour-633 (Invitrogen, A21052, IF: 1:500), Spy650-DNA (tebu-bio, sc501, 1:1000), Immu-Mount (Epredia, 9990402), Hoechst33258 (Sigma-Aldrich, 94403), Lipofectamine 3000 (Thermo Scientific, L3000001), NEBuilder HiFi°DNA°Assembly°Master Mix (NEB, E2621L), CloneAampTM HiFi PCR Premix (Takara Bio, 639298), GatewayTM BP ClonaseTM II AR TI CL E IN P RE SS Enzym-Mix (Thermo Scientific, 11789020), GatewayTM LR ClonaseTM II Enzym-Mix (Thermo Scientific, 11791020), AflII (New England Biolabs, R0520S), Polymerase Chain Reaction:Article Title: Screening assay to monitor mono-ADP-ribosylhydrolase activity of viral macrodomains in cells Article Snippet: The following reagents were used in this study: ß-NAD + (Sigma-Aldrich, N6522), 32 P-NAD + (Hartmann, FP-321), Protease inhibitor cocktail (Sigma-Aldrich, P8340; MedChem Express, HY-K0011), polybrene (Hexadimethrinbromid, Sigma Aldrich, 107689), doxycycline (Sigma-Aldrich, D9891), Z8539_0023 (Enamine-ID: Z5010894404; 4-hydroxy-1-{4-[(phenylcarbamoyl)amino]benzamido}-2.3-dihydro-1H-indene-2-carboxylic acid), Z8539_0029 (Enamine-ID: Z5010894407; 1-{4-[(cyclopropylcarbamoyl)amino]benzamido}-2,3-dihydro-1H-indene-2-carboxylic acid), Z3122 (Enamine-ID: Z5389393122; 3-(4-bromophenyl)-3-({7H-pyrrolo[2,3-d]pyrimidin-4-yl}formamido)propanoic acid), LL1_0023 (Enamine-ID: Z5030903496, 8-fluoro-6-{7-fluoro-9H-pyrimido[4,5-b]indol-4-yl}-6-azaspirol[3.4]octane-8-carboxylic acid), LRH-0003 (Enamine-ID: Z5265428218, 1-({7-fluoro-9H-pyrimido[4,5-b]indol-4-yl}amino)pyrrolodin-2-one), Z8539 (Enamine-ID: Z4718398539, 1-{4-[(cyclopropylcarbamyol)amino]benzamido}-4-hydroxy-2,3-dihydro-1H-indene-2-carboxylic acid) ; OUL232 (MedChem express HY-148566), OUL 243 ; GS441524 (Tocris, #7227, (2 R ,3 R ,4 S ,5 R )-2-(4-Aminopyrrolo[2,1- f ][1,2,4]triazin-7-yl)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-carbonitrile); MDOLL-0229 ; the pyrollo-pyrimidine-based compounds (7 H -pyrrolo[2,3- d ]pyrimidin-4-yl)- L -tryptophanate ( 5a ), (7 H -pyrrolo[2,3- d ]pyrimidin-4-yl)- L -tryptophanate ( 5c ), ( S )-2-((7 H -pyrrolo[2,3- d ]pyrimidin-4-yl)amino)- N -((2-chloropyridin-4-yl)methyl)-3-(1 H -indol-3-yl)propenamide ( 6b ), ( S )-2-((7 H -pyrrolo[2,3- d ]pyrimidin-4-yl)amino)-3-(1 H -indol-3-yl)- N -(pyridin-2-ylmethyl)propenamide ( 6d ), ( R )-2-((7 H -pyrrolo[2,3- d ]pyrimidin-4-yl)amino)-3-(1 H -indol-3-yl)- N -(pyridin-2-ylmethyl)propenamide ( 6e ) . .. TALON metal affinity resin (Takara, 635501), anti-GFP (Rockland, 600-101-2015, WB: 1:2000), anti-gamma-Tubulin (Sigma-Aldrich, GTU88, WB: 1:2000), anti-GAPDH (Santa Cruz, sc-32233, WB 1:2000), anti-PARP15 (Proteintech, 18126-1-AP, WB: 1:2000), anti-FLAG (Sigma Aldrich, F3165, clone M2, WB: 1:5000, IF: 1:500), goat-anti-mouse-HRP (Jackson Immunoresearch, 115-036-068, WB: 1:10000), rabbit-anti-goat-HRP (Santa Cruz, sc-2768, WB: 1:5000), goat-anti-rabbit IgG (Cell Sinalling, 7074P2, WB: 1:2000), goat-anti-mouse-Alexa-Flour-633 (Invitrogen, A21052, IF: 1:500), Spy650-DNA (tebu-bio, sc501, 1:1000), Immu-Mount (Epredia, 9990402), Hoechst33258 (Sigma-Aldrich, 94403), Lipofectamine 3000 (Thermo Scientific, L3000001), NEBuilder HiFi°DNA°Assembly°Master Mix (NEB, E2621L), CloneAamp TM HiFi PCR Premix (Takara Bio, 639298), GatewayTM BP ClonaseTM II Enzym-Mix (Thermo Scientific, 11789020), GatewayTM LR ClonaseTM II Enzym-Mix (Thermo Scientific, 11791020), AflII (New England Biolabs, R0520S), Article Title: Screening assay to monitor mono-ADP-ribosylhydrolase activity of viral macrodomains in cells. Article Snippet: The following reagents were used in this study: ß-NAD+ (Sigma-Aldrich, N6522), 32P-NAD+ (Hartmann, FP-321), Protease inhibitor cocktail (Sigma-Aldrich, P8340; MedChem Express, HYK0011), Polybrene (Hexadimethrinbromid, Sigma Aldrich, 107689), Doxycycline (Sigma-Aldrich, D9891), Z8539_0023 (Enamine-ID: Z5010894404; 4-hydroxy-1-{4[(phenylcarbamoyl)amino]benzamido}-2.3-dihydro-1H-indene-2-carboxylic acid), Z8539_0029 (Enamine-ID: Z5010894407; 1-{4-[(cyclopropylcarbamoyl)amino]benzamido}-2,3-dihydro-1Hindene-2-carboxylic acid), Z3122 (Enamine-ID: Z5389393122; 3-(4-bromophenyl)-3-({7H- AR TI CL E IN P RE SS pyrrolo[2,3-d]pyrimidin-4-yl}formamido)propanoic acid), LL1_0023 (Enamine-ID: Z5030903496, 8-fluoro-6-{7-fluoro-9H-pyrimido[4,5-b]indol-4-yl}-6-azaspirol[3.4]octane-8-carboxylic acid), LRH-0003 (Enamine-ID: Z5265428218, 1-({7-fluoro-9H-pyrimido[4,5-b]indol-4yl}amino)pyrrolodin-2-one), Z8539 (Enamine-ID: Z4718398539, 1-{4- [(cyclopropylcarbamyol)amino]benzamido}-4-hydroxy-2,3-dihydro-1H-indene-2-carboxylic acid)25; OUL232 (MedChem Express HY-148566), OUL 243 40; GS441524 (Tocris, #7227, (2R,3R,4S,5R)-2-(4-Aminopyrrolo[2,1-f][1,2,4]triazin-7-yl)-3,4-dihydroxy-5(hydroxymethyl)tetrahydrofuran-2-carbonitrile); MDOLL-0229 47; the pyrollo-pyrimidine-based compounds (7H-pyrrolo[2,3-d]pyrimidin-4-yl)-L-tryptophanate (5a), (7H-pyrrolo[2,3-d]pyrimidin4-yl)-L-tryptophanate (5c), (S)-2-((7H-pyrrolo[2,3-d]pyrimidin-4-yl)amino)-N-((2-chloropyridin-4yl)methyl)-3-(1H-indol-3-yl)propenamide (6b), (S)-2-((7H-pyrrolo[2,3-d]pyrimidin-4-yl)amino)-3(1H-indol-3-yl)-N-(pyridin-2-ylmethyl)propenamide (6d), (R)-2-((7H-pyrrolo[2,3-d]pyrimidin-4yl)amino)-3-(1H-indol-3-yl)-N-(pyridin-2-ylmethyl)propenamide (6e) 30. .. TALON metal affinity resin (Takara, 635501), anti-GFP (Rockland, 600-101-2015, WB: 1:2000), anti-gamma-Tubulin (SigmaAldrich, GTU88, WB: 1:2000), anti-GAPDH (Santa Cruz, sc-32233, WB 1:2000), anti-PARP15 (Proteintech, 18126-1-AP, WB: 1:2000), anti-FLAG (Sigma Aldrich, F3165, clone M2, WB: 1:5000, IF: 1:500), goat-anti-mouse-HRP (Jackson Immunoresearch, 115-036-068, WB: 1:10000), rabbitanti-goat-HRP (Santa Cruz, sc-2768, WB: 1:5000), goat-anti-rabbit IgG (Cell Sinalling, 7074P2, WB: 1:2000), goat-anti-mouse-Alexa-Flour-633 (Invitrogen, A21052, IF: 1:500), Spy650-DNA (tebu-bio, sc501, 1:1000), Immu-Mount (Epredia, 9990402), Hoechst33258 (Sigma-Aldrich, 94403), Lipofectamine 3000 (Thermo Scientific, L3000001), NEBuilder HiFi°DNA°Assembly°Master Mix (NEB, E2621L), CloneAampTM HiFi PCR Premix (Takara Bio, 639298), GatewayTM BP ClonaseTM II AR TI CL E IN P RE SS Enzym-Mix (Thermo Scientific, 11789020), GatewayTM LR ClonaseTM II Enzym-Mix (Thermo Scientific, 11791020), AflII (New England Biolabs, R0520S), Article Title: Baseline cellular state dictates the molecular impact of KRAS mutant variants in pancreatic cancer cells Article Snippet: The PGK promoter and GFP cassette were PCR-amplified from the MSCV Puro-pGK:GFP construct (Addgene #68486) and inserted into the linearized lentiviral backbone using Gibson Assembly (New England Biolabs (NEB), #E2611) as previously described ( ). .. KRAS mutant cDNA sequences were PCR-amplified from the RAS Mutant Clone Collection (Addgene, Kit #1000000089) with Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0494S) using forward primers containing a Plasmid Preparation:Article Title: Comprehensive CRISPR/Cas9-based mutagenesis identifies single-amino acid substitutions that abrogate SPEN function in X inactivation. Article Snippet: .. The insert, from dCas9 plasmid (Addgene #68416) and vector fragments were obtained by digesting both plasmids with 1 μL Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus Article Snippet: .. The plasmids were digested with SpeI-HF (NEB, R3133S) and Mutagenesis:Article Title: Baseline cellular state dictates the molecular impact of KRAS mutant variants in pancreatic cancer cells Article Snippet: The PGK promoter and GFP cassette were PCR-amplified from the MSCV Puro-pGK:GFP construct (Addgene #68486) and inserted into the linearized lentiviral backbone using Gibson Assembly (New England Biolabs (NEB), #E2611) as previously described ( ). .. KRAS mutant cDNA sequences were PCR-amplified from the RAS Mutant Clone Collection (Addgene, Kit #1000000089) with Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0494S) using forward primers containing a Clone Assay:Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus Article Snippet: .. The plasmids were digested with SpeI-HF (NEB, R3133S) and |